It has known that some strains of Enterococcus faecalis inhibit some strains of enterococci and group D streptococcal species by production of enterocin. We found that Clostridium perfringens did not grow in our previous report of 'In vivo
efficacy
of
cefotetan and cefoxitin in an intraabdominal abscess model in the mouse'. So we designed our experiments in vivo and in vitro to find out what bacteria inhibit the growth of C. perfringens.
@ES The results were as follows;
@EN 1) C. perfringens from the intraabdominal and the subcutaneous abscess model using 5 bacterial isolates and autoclaved cecal content were not found. In vitro studies with various combination of organisms, c. perfringens did not grow in the
groups of
the co-culture with E. faecalis.
2) In vivo models using E. faecalis (105/ml)and C. perfringens (105/ml), we couldn't find any colonies of C. perfringens although we used a more concentration of C. perfringens than E. faecalis.
3) In direct and deferred antogonism test, inhibition zone to C. perfringens by E.faecalis was found, and more concentration of E. faecalis was necessary for inhibition of C. perfringens.
4) The inhibiton zone by E. faecalis filtrates using 0.22um filter was also found, but we couldn't see any inhibition when we diluted the E. faecalis fitrates.
5) C. septicum, C. cadaveris, and C. subterminale were inhibited by E. faecalis, whereas C. difficile, and C. sporogenes were not inhibited
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